Answers is the place to go to get the answers you need and to ask the questions you want. A business day is generally considered 9 a.m. to 5 p.m. local time on weekdays (excluding federal holidays), not weekends. That means if you deposit a 100 check in your bank account at 11 a.m. on Friday, and that check is drawn on an account at the same bank, the next day your deposit could be available would be Monday (a business day) not.